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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
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Genome microsatellite diversity within the Apicomplexa phylum.
Juan Pablo Isaza1,2, Juan Fernando Alzate3,4
1Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Molecular Genetics and Genomics : MGG
|September 4, 2016
Summary
Single sequence repeats (SSRs) in Apicomplexa genomes show varied distribution. Di-nucleotide repeats are common in intergenic areas, while tri-nucleotide repeats are found in exons.
Area of Science:
- Genomics
- Parasitology
- Molecular Biology
Background:
- Apicomplexa are unicellular, obligate intracellular protozoan parasites.
- These parasites possess an apical complex crucial for host cell attachment and invasion.
- Understanding their genomic composition is key to parasite biology.
Purpose of the Study:
- To analyze the distribution and types of single sequence repeats (SSRs) across the genomes of 20 Apicomplexa species.
- To investigate the association between SSR types and their genomic locations (exon, intron, intergenic).
Main Methods:
- Whole-genome analysis of 20 Apicomplexa species.
- Identification of perfect SSRs (2-6 mers, ≥12 nucleotides).
- Classification of SSRs based on genomic location.
Main Results:
- SSR density varied significantly among the studied Apicomplexa genomes.
- Di-nucleotide and tri-nucleotide repeats were the most frequent SSR types.
- Di-nucleotide repeats were predominantly found in intergenic regions.
- Tri-nucleotide repeats were predominantly found in exon regions.
Conclusions:
- SSR distribution and type are not uniform across Apicomplexa genomes.
- Specific SSR types show distinct genomic region preferences within Apicomplexa.
- These findings contribute to understanding Apicomplexa genome organization and evolution.
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