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Pull-down method to access the cell surface receptor for Toxoplasma gondii
Haiyan Gong1, Kyousuke Kobayashi1, Tatsuki Sugi2
1Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Parasitology International
|September 4, 2016
Abstract:
Protein X, which is expressed on the surface of Toxoplasma and is thought to interact with host molecules, was expressed as a GST recombinant protein, conjugated to sepharose 4B, and used to pull down biotin-labeled 293T cells. The product was analysis by 2D-PAGE and Western blotting. Mass spectrometry revealed the reacted spots from the gel to be heat shock proteins.
