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Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
A naphthalene-based two-photon fluorescent probe for selective and sensitive detection of endogenous hypochlorous
Xiao-Hong Zhou1, Yu-Ren Jiang2, Xiong-Jie Zhao2
1College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083 China; Environment Monitoring Department, Changsha Environmental Protection College, Changsha, 410004 China.
Abstract:
An efficient naphthalene-based two-photon fluorescent probe for endogenous HClO has been reported in the present study, which consists of a 6-(2-benzothiazolyl)-2-naphthalenol fluorophore connected with a 4-aminophenol (the fluorescence quenching and response group). This probe exhibits a high selectivity and excellent sensitivity with a detection limit of 7.6nM over other reactive oxygen species and analyte species, and the fluorescence intensity enhanced 103-fold when responsed. Furthermore, it was successfully used for two-photon imaging of endogenous HClO in live cells with high-resolution.

