Effect of subchronic corticosterone administration on α₂-adrenoceptor functionality in rat brain: an in vivo and in...

Noradrenergic system plays a critical role in the hypothalamic-pituitary-adrenal (HPA) axis regulation and the stress response. A dysregulated HPA axis may be indicative of an increased biological vulnerability for depression. In addition, a variety of studies have focused on specific alterations of α₂-adrenoceptors as a mechanism involved in the pathogenesis of mood disorders and antidepressant response.

This study aimed to evaluate the effect of subchronic corticosterone administration on rat brain α₂-adrenoceptor functionality by in vitro [³⁵S]GTPγS binding stimulation assays and in vivo dual-probe microdialysis determination of extracellular noradrenaline concentrations.

Implantation of a time release corticosterone pellet during 14 days induced sustained changes in endocrine function. However, there were no differences in α₂-adrenoceptor agonist UK14304-induced stimulation of [³⁵S]GTPγS binding in prefrontal cortex (PFC) between corticosterone-treated and control rats. In the same way, the in vivo evaluation of α₂-adrenoceptor-mediated noradrenaline release responses to the α₂-adrenoceptor agonist clonidine local administration into the locus coeruleus (LC), and the PFC did not show differences between the groups.

The present results show that subchronic corticosterone administration does not induce changes on functionality of α₂-adrenoceptors neither in the LC nor in noradrenergic cortical terminal areas.