Related Experiment Videos
Radiation inactivation analysis of the A1 adenosine receptor of rat brain. Decrease in radiation inactivation size in
M Reddington1, K N Klotz, M J Lohse
1Department of Neuromorphology, Max Planck Institute for Psychiatry, Martinsried, FRG.
FEBS Letters
|July 31, 1989
Abstract:
Radiation inactivation analysis of the binding of the A1 adenosine receptor antagonist, 8-cyclopentyl-1,3-dipropylxanthine to rat brain membranes yielded a radiation inactivation size of 58 kDa. In the presence of GTP gamma S this was reduced to 33 kDa, in good agreement with the size of the ligand-binding subunit detected after photoaffinity labelling. The data indicate that the structural association of A1 adenosine receptors with G-protein components is altered in situ in the presence of guanine nucleotides.