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A rapid method to estimate NADH regeneration rates in living cells
Jannis Kuepper1, Sebastian Zobel1, Nick Wierckx1
1Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Journal of Microbiological Methods
|September 6, 2016
Abstract:
We introduce a rapid whole cell assay for the estimation of NADH regeneration rates based on the fluorescent dye resazurin. A co-feed of formate and glucose, known to increase the intrinsic NADH regeneration rate of P. putida KT2440, was chosen as model system for the validation of this assay.
