A rapid method to estimate NADH regeneration rates in living cells
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Jannis Kuepper1, Sebastian Zobel1, Nick Wierckx1
1Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Journal of Microbiological Methods
|September 6, 2016
Abstract:
We introduce a rapid whole cell assay for the estimation of NADH regeneration rates based on the fluorescent dye resazurin. A co-feed of formate and glucose, known to increase the intrinsic NADH regeneration rate of P. putida KT2440, was chosen as model system for the validation of this assay.
