Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mate Choice

Mate Choice

Mate choice—the decision about whom to mate with—is a type of natural selection, since animals must reproduce to pass down their genes. Mate choice is also called intersexual selection because the behavior occurs between the sexes.

Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genomic outbreak investigation of biosafety-level-3 pathogens using nanopore sequencing.

Microbial genomics·2026

Same author

Biofertilizers.

Journal of basic microbiology·2026

Same author

Tuning reductase activity in monoterpene indole alkaloid biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Comparative proteome analyses of human natural killer cells from patients after allogeneic stem cell transplantation and healthy individuals challenged with <i>Aspergillus fumigatus</i>.

microLife·2026

Same author

Organic Molecules are Involved in Ni-Struvite Biomineralization by Streptomyces mirabilis.

Molecular biotechnology·2026

Same author

Inducible gene deletion reveals essentiality of protein kinases and a septation initiation network in Candida albicans.

PLoS genetics·2026

Search research articles

Related Experiment Video

Updated: Mar 15, 2026

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

Published on: July 17, 2017

Smelling the difference: Transcriptome, proteome and volatilome changes after mating.

Daniela Freihorst¹, Melanie Brunsch¹, Sophia Wirth¹

¹Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.

Fungal Genetics and Biology : FG & B

|September 6, 2016

Summary

This summary is machine-generated.

Mating in Schizophyllum commune mushrooms triggers significant changes in gene expression and protein regulation. This interaction leads to altered volatile compound production, enhancing the characteristic mushroom odor.

Keywords:

Basidiomycete Mating Proteome Schizophyllum commune Transcriptome Volatilome

More Related Videos

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Published on: August 9, 2024

Related Experiment Videos

Last Updated: Mar 15, 2026

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

Published on: July 17, 2017

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Published on: August 9, 2024

Area of Science:

Mycology
Molecular Biology
Biochemistry

Background:

Mushrooms possess distinct odors, but the link between odor production and mating processes, essential for mushroom formation, remains unclear.
Investigating odor development in both monokaryotic (unmated) and dikaryotic (mated) fungal strains is crucial for understanding fungal development.

Purpose of the Study:

To investigate the relationship between mating, odor production, and the underlying molecular mechanisms in the model mushroom Schizophyllum commune.
To compare transcriptional and proteomic changes during mating and correlate them with volatile compound emissions.

Main Methods:

Comprehensive transcriptome and proteome analysis of monokaryotic and dikaryotic mycelia.
Volatilome studies to analyze changes in emitted volatile organic compounds.
Comparison of proteomic, transcriptomic, and volatilomic data to identify regulatory networks.

Main Results:

A strong correlation was observed between transcriptome and proteome data, with over 80% of transcripts showing post-translational regulation.
Mating significantly broadened the spectrum of volatile compounds and increased the emission of key odorants like 3-octanone and 1-octen-3-ol.
Transcriptional regulation of key biosynthetic enzymes, including alcohol dehydrogenases and sesquiterpene synthases, varied between monokaryotic and dikaryotic stages.

Conclusions:

Fungal mating involves extensive post-translational regulation, influencing downstream processes and dikaryon formation.
Mating-induced changes in volatile compounds contribute to the characteristic mushroom odor.
Specific transcriptional patterns of biosynthetic genes are associated with different mating stages, highlighting stage-specific metabolic regulation.