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Fast biosensor-based system for measuring total DNA in protein solutions.

J Briggs¹, R Zuk

¹Molecular Devices Corporation, Palo Alto, California 94304.

Developments in Biological Standardization

|January 1, 1989

Summary

This summary is machine-generated.

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This study introduces a rapid, non-isotopic assay for quantifying picogram levels of DNA. The system uses DNA-binding proteins and a biosensor for sensitive detection of any DNA sample.

Area of Science:

Biotechnology
Analytical Chemistry
Molecular Biology

Background:

Accurate DNA quantitation is crucial for various biological and diagnostic applications.
Existing methods may be time-consuming, require isotopes, or lack sensitivity for low DNA amounts.

Purpose of the Study:

To develop and report a novel, fast, and reproducible assay for sensitive DNA quantitation.
To demonstrate a generic DNA detection system applicable to various samples.

Main Methods:

Utilized two DNA-binding proteins for sample capture and enzyme labeling.
Employed a proprietary biosensor to monitor surface potential changes via enzyme-induced pH shifts.
Developed a non-isotopic assay system for high-sensitivity detection.

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Main Results:

Achieved quantitation of picogram levels of DNA (2-100 pg) with high sensitivity.
Demonstrated a generic assay capable of detecting any DNA without specific probes.
Completed multi-sample analysis with standards in under one hour post-pretreatment.

Conclusions:

The developed assay system offers a fast, reproducible, and sensitive method for DNA quantitation.
The generic nature and speed make it suitable for various analytical biotechnology applications.
The prototype system integrates instrumentation, software, disposables, and reagents for a complete solution.