Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

15.9K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
15.9K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.6K
4.6K
Homologous Recombination02:31

Homologous Recombination

65.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baseline levels and dynamic trajectories of fasting C peptide to blood glucose ratio predict the risk of combined vascular damage in type 2 diabetes: a longitudinal cohort study.

Diabetes research and clinical practice·2026
Same author

The Case | Progressive submandibular mass and nephrotic syndrome in a young man.

Kidney international·2026
Same author

Microbial inoculations enhance soil aggregation and carbon stabilization via root exudate-mediated microbial association networks.

The New phytologist·2026
Same author

Novel ABO*B allele (c.701C>T) on ABO*B.01 in a Chinese B(A) phenotype individual.

Transfusion·2026
Same author

The effect of endophytic bacteria on the growth, medicinal quality, and rhizosphere soil environment of <i>Isatis indigotica</i> Fort.

Frontiers in plant science·2026
Same author

Habitat suitability assessment of wild medicinal plant resources in the Changbai Mountain ecosystem of China Using MaxEnt and AHP.

Frontiers in plant science·2026

Related Experiment Video

Updated: Mar 15, 2026

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells

Published on: September 8, 2010

32.9K

GUIDE-Seq to Detect Genome-wide Double-Stranded Breaks in Plants.

Lina Shi1, Xiaoqing Tang2, Guiliang Tang1

  • 1Provincial State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.

Trends in Plant Science
|September 6, 2016
PubMed
Summary

DNA damage and repair occur in plant and animal cells. We highlight GUIDE-seq, a therapeutic technology, and propose its use in plants for DNA repair studies.

Keywords:
BLESSCRISPR/Cas9DNA damage repair/responseGUIDE-seqHTGTSIDLV capturePTO linkagedigenome-seqdouble-stranded breaksdouble-stranded oligonucleotidesingle-stranded oligonucleotide

More Related Videos

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response
05:01

Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response

Published on: June 14, 2024

2.4K

Related Experiment Videos

Last Updated: Mar 15, 2026

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells

Published on: September 8, 2010

32.9K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response
05:01

Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response

Published on: June 14, 2024

2.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Animal and plant cells possess intrinsic DNA damage repair mechanisms.
  • Understanding DNA damage and repair dynamics is crucial for cellular health and therapeutic development.

Purpose of the Study:

  • To highlight the GUIDE-seq technology for its application in studying DNA repair.
  • To explore the potential of GUIDE-seq in plant systems for DNA damage and repair analysis.

Main Methods:

  • Review of existing technologies for DNA damage and repair assessment, including IDLV capture, BLESS, HTGTS, digenome-seq.
  • Focus on Genome-wide Unbiased Identification of DSBs Enabled by Sequencing (GUIDE-seq) technology.

Main Results:

  • GUIDE-seq is a powerful tool for identifying DNA double-strand breaks (DSBs) with high accuracy.
  • The technology has demonstrated utility in therapeutic applications for genome editing assessment.

Conclusions:

  • GUIDE-seq offers a promising approach for investigating DNA damage and repair in plants.
  • Adapting GUIDE-seq for plant research could advance our understanding of plant genome stability and response to damage.