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Updated: Mar 15, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
Suppression of MicroRNA let-7a Expression by Agmatine Regulates Neural Stem Cell Differentiation
Juhyun Song1, Yumi Oh1,2, Jong Youl Kim1
1Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea.
Purpose:
Neural stem cells (NSCs) effectively reverse some severe central nervous system (CNS) disorders, due to their ability to differentiate into neurons. Agmatine, a biogenic amine, has cellular protective effects and contributes to cellular proliferation and differentiation in the CNS. Recent studies have elucidated the function of microRNA let-7a (let-7a) as a regulator of cell differentiation with roles in regulating genes associated with CNS neurogenesis.
Materials And Methods:
This study aimed to investigate whether agmatine modulates the expression of crucial regulators of NSC differentiation including DCX, TLX, c-Myc, and ERK by controlling let-7a expression.
Results:
Our data suggest that high levels of let-7a promoted the expression of TLX and c-Myc, as well as repressed DCX and ERK expression. In addition, agmatine attenuated expression of TLX and increased expression of ERK by negatively regulating let-7a.
Conclusion:
Our study therefore enhances the present understanding of the therapeutic potential of NSCs in CNS disorders.
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