Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Esophageal microbiome correlates with post-esophagectomy anastomotic leak in cancer patients.

ESMO gastrointestinal oncology·2026
Same author

Multidisciplinary discussion in upper GI tumours: management of a patient with a locally advanced Siewert II gastroesophageal junctional adenocarcinoma.

ESMO gastrointestinal oncology·2026
Same author

Multidisciplinary discussion on upper GI tumours: management of a patient with a middle-third locally advanced squamous-cell carcinoma.

ESMO gastrointestinal oncology·2026
Same author

The evolving landscape of leptomeningeal metastases from NSCLC: an international, contemporary, multicenter cohort study.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Pain management after minimally invasive gastrectomy: do we need epidural analgesia?

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2025
Same author

Curative treatment for oligometastatic gastroesophageal cancer- results of a prospective multicenter study.

Langenbeck's archives of surgery·2024

Related Experiment Video

Updated: Mar 15, 2026

Author Spotlight: An Innovative Approach to Neural Electrical Stimulation Using Calcium Imaging
07:25

Author Spotlight: An Innovative Approach to Neural Electrical Stimulation Using Calcium Imaging

Published on: August 18, 2023

2.7K

Scintillate: An open-source graphical viewer for time-series calcium imaging evaluation and pre-processing.

I A N Dublon1, M Nilsson2, A Balkenius1

  • 1Division of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, P.O. Box 102, SE-230 53, Alnarp, Sweden.

Journal of Neuroscience Methods
|September 6, 2016
PubMed
Summary

This study introduces scintillate, an open-source software for rapid analysis of calcium imaging data. It enables whole-image fluorescence change (ΔF) and Independent Component Analysis (ICA) for efficient sample evaluation and acquisition optimization.

Keywords:
Calcium imagingFluorescenceGUIICAPre-processingoptical imaging

More Related Videos

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.8K
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
09:39

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

Published on: December 16, 2022

5.2K

Related Experiment Videos

Last Updated: Mar 15, 2026

Author Spotlight: An Innovative Approach to Neural Electrical Stimulation Using Calcium Imaging
07:25

Author Spotlight: An Innovative Approach to Neural Electrical Stimulation Using Calcium Imaging

Published on: August 18, 2023

2.7K
Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.8K
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
09:39

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

Published on: December 16, 2022

5.2K

Area of Science:

  • Neuroscience
  • Biophysics
  • Computational Biology

Background:

  • Calcium imaging detects subtle signal changes in image time-series.
  • Analysis often requires post-processing of large datasets and Region of Interest (ROI) selection.
  • Responses can range from pronounced (genetically encoded reporters) to subtle (bath-applied dyes).

Purpose of the Study:

  • To introduce scintillate, a novel software for real-time evaluation of calcium imaging data.
  • To enable rapid assessment of image data without a priori ROI selection.
  • To improve the efficiency of experimental design and data acquisition.

Main Methods:

  • Developed scintillate, a GUI-based platform for viewing time-sequenced tiff images.
  • Implemented whole-image fluorescence change (ΔF) analysis.
  • Integrated Independent Component Analysis (ICA) for signal change detection.

Main Results:

  • Demonstrated scintillate's ability to evaluate both strong genetically encoded neuronal Ca2+ responses in flies and weaker dye-based responses in moth mushroom bodies.
  • Showcased instantaneous pre-stimulation background subtraction for improved signal appraisal.
  • Validated the utility of whole-matrix ΔF and ICA for rapid sample assessment and acquisition optimization.

Conclusions:

  • Scintillate provides a multi-platform, open-source system for efficient time-series image evaluation.
  • The software minimizes time spent on unresponsive samples and aids in optimizing subsequent acquisitions.
  • Whole-matrix ΔF and ICA analysis at the time of acquisition are invaluable for calcium imaging experiments.