Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

71
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
71
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.4K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.4K
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

79
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
79
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

112.9K
Overview
112.9K
Modified-Release Drug Delivery Systems: Stimuli-Activated01:30

Modified-Release Drug Delivery Systems: Stimuli-Activated

73
Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also...
73
Facilitated Diffusion01:16

Facilitated Diffusion

1.5K
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathway-Aware Template-Based Retrosynthesis.

Journal of chemical information and modeling·2026
Same author

A complete human pancreatic cancer genome.

bioRxiv : the preprint server for biology·2026
Same author

Deletion of low-essentiality, secretion-associated genes enhances recombinant protein production in Komagataella phaffii.

Microbial cell factories·2026
Same author

A 3D In Vitro Model of the Human Hepatobiliary Junction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

ASO Visual Abstract: Colloid Carcinoma of the Pancreas: A Distinct and Less Aggressive Entity in the Spectrum of Pancreatic Malignancies.

Annals of surgical oncology·2026
Same author

Colloid Carcinoma of the Pancreas: A Distinct and Less Aggressive Entity in the Spectrum of Pancreatic Malignancies.

Annals of surgical oncology·2026
Same journal

Anion-Engineered Organic Electrochemical Transistors With Multi-Timescale Synaptic Dynamics for Task-Adaptive Spiking Neural Networks.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Dimensional Effect on the Lattice Anharmonicity in Graphene and Graphite.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

A Modular Core-Shell Nanoparticle Platform for Dual-Modal MRI-Luminescence With High Relaxivity.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Highly Selective Construction of D<sub>2</sub>-Symmetric Chiral Carbon Nanorings and the Diverse Assembly With Fullerenes.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

A Synergistic Process Optimization and Data-Driven Modeling Strategy for Unraveling and Enhancing the Low-Light Response in Back-Contact Solar Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Porous Hydrogel-Mediated One-Step Selection of Mannoprotein-Targeted Aptamers for Early Diagnosis of Invasive Saccharomyces cerevisiae Infections.

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Mar 15, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.4K

A Size-Selective Intracellular Delivery Platform.

May Tun Saung1,2, Armon Sharei1, Viktor A Adalsteinsson1

  • 1Department of Chemical Engineering, Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|September 6, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic device that uses cell size to selectively deliver cargo into specific cells. This method enables the isolation and genomic analysis of circulating tumor cells from patient blood samples.

Keywords:
circulating tumor cellsintracellular deliverymicrofluidicssize-selective delivery

More Related Videos

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

9.0K
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

10.5K

Related Experiment Videos

Last Updated: Mar 15, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.4K
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

9.0K
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

10.5K

Area of Science:

  • Cell Biology
  • Biotechnology
  • Microfluidics

Background:

  • Cell separation and intracellular delivery are crucial in biological research.
  • Existing methods often require prior knowledge of specific cell surface markers.
  • A novel approach is needed for high-throughput, marker-independent cell manipulation.

Purpose of the Study:

  • To develop a microfluidic device for size-selective intracellular delivery.
  • To demonstrate the ability to differentiate and deliver cargo to cells of varying sizes.
  • To enable the isolation and characterization of specific cell populations, such as circulating tumor cells.

Main Methods:

  • Utilized a high-throughput microfluidic device with adjustable constriction widths.
  • Exploited transient cell membrane disruption at constrictions for cytoplasmic cargo delivery.
  • Optimized constriction dimensions (e.g., 4 μm, 6-7 μm) for different cell sizes (T-cells, pancreatic cancer cells).

Main Results:

  • Achieved size-selective cargo delivery to primary human T-cells and pancreatic cancer cell lines.
  • Demonstrated successful differentiation of pancreatic cancer cells from T-cells based on size.
  • Successfully delivered fluorophores to circulating tumor cells in patient blood for subsequent isolation.

Conclusions:

  • Microfluidic-based size-selective intracellular delivery is a viable method for cell isolation.
  • This technique facilitates the analysis of specific cell subpopulations, including circulating tumor cells.
  • The approach offers a powerful tool for biological research and clinical diagnostics.