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NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression.

Anna Spina1, Roberta Montella1, Davide Liccardo1

  • 1Sezione di Biotecnologie, Dipartimento di Medicina Sperimentale, Istologia Medica e Biologia Molecolare, Seconda Università degli Studi di Napoli Napoli, Italy.

Frontiers in Physiology
|September 6, 2016
PubMed
Summary

New Zealand fetal bovine serum (NZ-FBS) supports human dental pulp stem cell (hDPSC) proliferation and osteogenic differentiation, crucial for bone tissue engineering. This safer serum maintains essential cell functions for clinical applications.

Keywords:
angiogenesisbioscaffoldbone differentiationhDPSCsosteogenesis

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Area of Science:

  • Stem Cell Biology
  • Tissue Engineering
  • Biomaterials

Background:

  • Human dental pulp stem cells (hDPSCs) are neural crest-derived ecto-mesenchymal stem cells vital for bone tissue engineering.
  • Current Good Manufacturing Practice (GMP) procedures are essential for clinical applications, necessitating alternatives to conventional fetal bovine serum (FBS) due to infection risks.

Purpose of the Study:

  • To evaluate the behavior of hDPSCs cultured with New Zealand FBS (NZ-FBS), a clinical-grade serum, as a safer alternative to conventional FBS.
  • To determine if hDPSCs retain their osteogenic and angiogenic differentiation capabilities when expanded in NZ-FBS.

Main Methods:

  • hDPSCs were cultured for 7, 14, and 21 days in 2D and 3D systems using either NZ-FBS or conventional FBS (C-FBS).
  • Comparative analysis included growth curves, expression of bone-related markers (BSP, OPN), calcification, and angiogenesis (VEGF, PDGFA) evaluation.
  • hDPSCs were also seeded on a clinical-grade collagen I scaffold (Bio-Gide®) to assess behavior in a relevant biomaterial context.

Main Results:

  • NZ-FBS significantly enhanced hDPSC proliferation compared to C-FBS.
  • Earlier upregulation of osteogenic markers (BSP, OPN) involved in mineralized matrix formation was observed with NZ-FBS within 14 days.
  • hDPSCs cultured in NZ-FBS exhibited higher mRNA levels of angiogenic factors (VEGF, PDGFA).
  • These beneficial effects were also observed when hDPSCs were cultured on a clinical-grade collagen I scaffold.

Conclusions:

  • hDPSCs cultured in NZ-FBS maintain robust proliferation, enhanced osteogenic commitment, and increased angiogenic factor production.
  • The use of GMP-approved materials, including NZ-FBS and clinical-grade scaffolds, does not negatively impact critical hDPSC functions for human therapy.
  • NZ-FBS represents a viable and safer alternative serum for expanding hDPSCs for therapeutic applications in bone tissue engineering.