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Published on: December 19, 2020
The Immunogenicity of Peptoid-Protein Conjugates
Allison Case1, Angela Desmond2, Daniel Lopes3
1Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Bioengineering, The University of Texas at Dallas, Texas, USA.
Abstract:
We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.
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