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High Resolution Melting (HRM) applied to wine authenticity.

Leonor Pereira1, Sónia Gomes1, Cláudia Castro1

  • 1University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, 1749-016 Lisboa, Portugal.

Food Chemistry
|September 7, 2016
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Summary

High Resolution Melting (HRM) assays offer a rapid and efficient method for verifying wine authenticity. This DNA-based technique successfully screened grape must and wine, ensuring varietal composition and supporting Denomination of Origin designations.

Keywords:
AuthenticityHRMMustVitis vinifera L.Wine

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Food Science

Background:

  • Growing demand for wine authenticity, particularly within Denomination of Origin (DO) systems.
  • DNA-based methods are crucial for accurate food and wine varietal tracking.
  • Need for efficient screening tools for wine and grape must authenticity.

Purpose of the Study:

  • To evaluate High Resolution Melting (HRM) as a screening method for must and wine authenticity.
  • To validate three developed HRM assays (Vv1-705bp, Vv2-375bp, Vv3-119bp) across different sample types.
  • To assess the suitability of HRM for ensuring wine varietal composition.

Main Methods:

  • Development and validation of three distinct High Resolution Melting (HRM) assays.
  • Application of HRM assays to three sample types: grape leaf, must, and wine.
  • Analysis of amplicon melting curves and temperatures for genotype discrimination.

Main Results:

  • The Vv2 HRM assay demonstrated successful amplification across all sample types (leaf, must, wine), enabling genotype differentiation via melting temperatures.
  • The Vv3 HRM assay, using the smallest amplicon, yielded consistent melting curve shapes across leaf and wine samples, correlating with genotypes.
  • The Vv1 HRM assay was effective for leaf and must but not for wine samples.

Conclusions:

  • Sensitive, rapid, and efficient HRM assays have been developed for wine authenticity screening.
  • This study marks the first application of HRM assays to wine samples for authenticity verification.
  • HRM technology shows significant potential for ensuring wine varietal integrity and supporting regulatory frameworks.