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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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CRISPR/Cas9 gene editing special issue.

John G Doench1

  • 1Broad Institute of MIT and Harvard, Cambridge, MA, USA.

The FEBS Journal
|September 7, 2016
PubMed
Summary
This summary is machine-generated.

This Special Issue features nine reviews on CRISPR technology, detailing its development and diverse applications across biological systems. These articles offer practical insights and thoughtful perspectives on CRISPR's potential.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has revolutionized biological research.
  • Its precise gene-editing capabilities offer unprecedented control over genetic material.

Purpose of the Study:

  • To provide a comprehensive overview of CRISPR technology.
  • To explore the development and application of CRISPR across various biological systems.

Main Methods:

  • This Special Issue compiles nine expert review articles.
  • The reviews synthesize current knowledge on CRISPR's evolution and use.

Main Results:

  • The collection covers CRISPR's development and application.
  • Insights into its utility in diverse biological contexts are presented.

Conclusions:

  • The reviews offer valuable, practical information on CRISPR.
  • This compilation serves as a helpful resource for researchers in the field.