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Updated: Mar 15, 2026
Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
Published on: October 3, 2019
miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene
Changping Ma1, Huibin Song1, Lei Yu1
1Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
Abstract:
A growing number of reports have revealed that microRNAs (miRNAs) play critical roles in spermatogenesis. Our previous study showed that miR-762 is differentially expressed in immature and mature testes of Large White boars. Our present data shows that miR-762 directly binds the 3' untranslated region (3'UTR) of ring finger protein 4 (RNF4) and down-regulates RNF4 expression. A single nucleotide polymorphism (SNP) in the RNF4 3'UTR that is significantly associated with porcine sperm quality traits leads to a change in the miR-762 binding ability. Moreover, miR-762 promotes the proliferation of and inhibits apoptosis in porcine immature Sertoli cells, partly by accelerating DNA damage repair and by reducing androgen receptor (AR) expression. Taken together, these findings suggest that miR-762 may play a role in pig spermatogenesis by regulating immature Sertoli cell growth.
