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A system for long-term corneal perfusion.

I Brunette1, L R Nelson, W M Bourne

  • 1Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota 55905.

Investigative Ophthalmology & Visual Science
|August 1, 1989
PubMed
Summary
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This study demonstrates a novel perfusion system for maintaining human corneas, preserving endothelial cell structure and function for up to three weeks. This method allows for controlled research into corneal preservation techniques.

Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Cell Biology

Background:

  • Human corneas require specific conditions for preservation.
  • Long-term ex vivo studies are crucial for understanding corneal health and developing new preservation methods.

Purpose of the Study:

  • To establish and validate a long-term human corneal perfusion system.
  • To assess the viability and structural integrity of corneas under perfusion.
  • To investigate the effects of specific perturbations on corneal endothelium in situ.

Main Methods:

  • Seventy-two human corneas were perfused at 37°C and 18 mm Hg for 1-3 weeks.
  • Corneal thickness, endothelial cell morphology, and ultrastructure were monitored.
  • Perturbations included endothelial removal, ouabain perfusion, and cold exposure.

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Main Results:

  • Corneal thickness slowly decreased during perfusion.
  • Endothelial removal or ouabain induced irreversible stromal swelling.
  • Cold-induced swelling was reversible, with less swelling after prolonged perfusion.
  • Electron microscopy confirmed reversal of postmortem changes and maintained ultrastructure for 3 weeks.

Conclusions:

  • The developed perfusion system effectively maintains human corneal viability and endothelial integrity ex vivo for up to three weeks.
  • This system provides a reliable platform for controlled in situ studies of corneal endothelium.
  • It facilitates research into novel corneal preservation strategies and the impact of various factors on corneal health.