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Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
Vanadate-induced antiproliferative and apoptotic response in esophageal squamous carcinoma cell line EC109
Jie Yang1, Zhuxia Zhang1, Shuyuan Jiang1
1a Biomedicine Research Center and Basic Medical College , Baotou Medical College , Inner Mongolia , P. R. China.
Abstract:
Vanadate is a transition element that present in nature and was shown to be a nonspecific inhibitor of protein tyrosine phosphatases. It was reported that vanadium (Vd) compounds exhibit antitumor actions in several cancer cell lines. This study aimed to examine the antiproliferative and apoptotic actions of different concentrations of sodium vanadate (NaVd) (+5) in esophageal squamous carcinoma cell line EC109 by determining the protein expression levels of cyclin D1 and caspase-3 following incubation for various times from 15 min up to 4 h. In addition, cell proliferation of EC109 treated with different concentrations (NaVd) was also measured using the MTT assay at 4, 12, 24, and 48 h. The cell cycle of EC109 cells exposed to different concentrations of NaVd was detected using flow cytometry determination at 24 h. Data showed that NaVd greater than 100 µM significantly increased cyclin D1. In contrast, reduced caspase-3 protein expression levels occurred at 50 µM. Cellular proliferation was significantly decreased at 50uM. The cell cycle was arrested at S phase with 100 µM NaVd. Taken together, data indicate that NaVd produced concentration- and time-dependent antitumor actions in EC109 cell line.
Insights
Sodium vanadate (NaVd) demonstrates antitumor effects in esophageal cancer cells. It impacts cell proliferation, apoptosis markers like caspase-3, and cell cycle progression, showing concentration-dependent activity.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Vanadate compounds are known inhibitors of protein tyrosine phosphatases.
- Vanadium compounds have demonstrated antitumor properties in various cancer cell lines.
Purpose of the Study:
- To investigate the antiproliferative and apoptotic effects of sodium vanadate (NaVd) on the EC109 esophageal squamous carcinoma cell line.
- To determine the impact of NaVd on cyclin D1 and caspase-3 protein expression, cell proliferation, and cell cycle progression.
Main Methods:
- Treatment of EC109 cells with varying concentrations of NaVd for different time points.
- Analysis of protein expression levels (cyclin D1, caspase-3) via Western blotting.
- Assessment of cell proliferation using the MTT assay.
- Cell cycle analysis using flow cytometry.
Main Results:
- NaVd at concentrations >100 µM significantly increased cyclin D1 expression.
- Caspase-3 protein levels were reduced at 50 µM NaVd.
- Cellular proliferation was significantly decreased at 50 µM NaVd.
- A cell cycle arrest at the S phase was observed with 100 µM NaVd.
Conclusions:
- Sodium vanadate exhibits concentration- and time-dependent antitumor activity in the EC109 esophageal squamous carcinoma cell line.
- NaVd influences key proteins involved in cell cycle regulation and apoptosis, suggesting its potential as an anticancer agent.
