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Updated: Mar 15, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
High speed color imaging through scattering media with a large field of view
Huichang Zhuang1, Hexiang He2, Xiangsheng Xie1,3
1State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Abstract:
Optical imaging through complex media has many important applications. Although research progresses have been made to recover optical image through various turbid media, the widespread application of the technology is hampered by the recovery speed, requirement on specific illumination, poor image quality and limited field of view. Here we demonstrate that above-mentioned drawbacks can be essentially overcome. The realization of high speed color imaging through turbid media is successfully carried out by taking into account the media memory effect, the point spread function, the exit pupil of the optical system, and the optimized signal to noise ratio. By retrieving selected speckles with enlarged field of view, high quality image is recovered with a responding speed only determined by the frame rates of the image capturing devices. The immediate application of the technique is expected to register static and dynamic imaging under human skin to recover information with a wearable device.
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