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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Mar 1, 2026

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

Assessing Bacterial Interactions Using Carbohydrate-Based Microarrays.

Andrea Flannery^1,2, Jared Q Gerlach^3,4,5, Lokesh Joshi^6,7

¹Carbohydrate Signalling Group, Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland. a.flannery5@nuigalway.ie.

Microarrays (Basel, Switzerland)

|September 8, 2016

Summary

Carbohydrate microarrays reveal how bacteria bind to hosts, aiding understanding of microbial pathogenesis and host immune responses. This technology is key to developing new therapeutic strategies against infectious diseases.

Keywords:

adhesins bacteria bacterial polysaccharides carbohydrate microarrays cross-talk glycan microarrays glycomics microorganisms mucins polysaccharides

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Last Updated: Mar 1, 2026

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Published on: April 5, 2015

Area of Science:

Microbiology
Glycoscience
Immunology

Background:

Carbohydrates are vital in host-microorganism interactions, mediating bacterial binding to host cells.
Host and bacterial glycosylation patterns influence species specificity and tropism of bacteria.
Understanding these interactions is crucial for deciphering microbial pathogenesis.

Purpose of the Study:

To review the application of carbohydrate-based microarrays in studying host-microorganism interactions.
To highlight the importance of characterizing specific carbohydrate structures in these interactions.
To explore how these microarrays can inform strategies against infectious diseases.

Main Methods:

Discussion of carbohydrate-based microarray platforms, including conventional glycan, whole mucin, and bacterial polysaccharide arrays.
Profiling of microarrays with whole bacteria, bacterial adhesins, and host serum antibodies.
Analysis of bacterial binding to diverse carbohydrate structures.

Main Results:

Carbohydrate microarrays are effective tools for screening bacterial interactions with specific host glycans.
Different microarray types provide distinct insights into host-microbe recognition.
These platforms facilitate the identification of key molecular players in host-pathogen adhesion.

Conclusions:

Carbohydrate microarrays are valuable for elucidating carbohydrate-mediated interactions in pathogenesis and immunity.
This technology can clarify mechanisms of infectious disease and host immune responses.
Findings may lead to novel therapeutic approaches targeting bacterial adhesion and host interactions.