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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Overview of Microscopy Techniques01:22

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function.

Isabell Begemann1, Milos Galic1

  • 1DFG Cluster of Excellence 'Cells in Motion', (EXC 1003), University of Muenster, MuensterGermany; Institute of Medical Physics and Biophysics, University Hospital Münster, University of Muenster, MuensterGermany.

Frontiers in Synaptic Neuroscience
|September 8, 2016
PubMed
Summary
This summary is machine-generated.

Correlative light electron microscopy (CLEM) bridges functional imaging and ultrastructural details. This technique revisits neurobiological questions about structure-function relationships in neurons and synapses.

Keywords:
CLEMSEMTEMcorrelative light electron microscopyelectron microscopyfluorescence microscopyneuronsynapse

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Area of Science:

  • Neuroscience
  • Microscopy
  • Cell Biology

Background:

  • Neuroscience relies heavily on light and electron microscopy.
  • These techniques are traditionally viewed as separate.
  • Advancements enable combining their strengths.

Purpose of the Study:

  • Review developments in microscopy driving correlative approaches.
  • Highlight current correlative light electron microscopy (CLEM) techniques.
  • Discuss CLEM's potential and limitations for neuronal applications.

Main Methods:

  • Correlating functional fluorescence microscopy data.
  • Acquiring ultrastructural information from electron micrographs.
  • Utilizing advancements in microscopy, labeling, and preparation.

Main Results:

  • Hybrid approaches now link functional and ultrastructural data.
  • CLEM enables revisiting structure-function relationships.
  • Various CLEM techniques are becoming accessible.

Conclusions:

  • CLEM offers powerful insights into neuronal and synapse biology.
  • The technique has potential and limitations that need consideration.
  • Further development will enhance its utility in neuroscience.