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Published on: March 1, 2013
Vitamin E-Labeled Polyethylenimine for in vitro and in vivo Gene Delivery
Jinxing Liu1, Mengke Feng1, Duanwei Liang1
1State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Abstract:
A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell lines. PEI-vitE6 showed the best performance in gfp pDNA delivery and following GFP expression in HEK-293A cells. In addition, in vivo gene delivery in living mice also confirmed that PEI-vitE6 showed low toxicity and efficiently delivered gfp pDNA to the cells of liver and lung tissues for gene expression.
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