Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

24.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-Dimensional Perfusion Imaging of Microcirculatory Networks Within Axially Vascularized Artificial Tissue.

Tissue engineering. Part C, Methods·2026
Same author

Deciphering secondary metabolite potentials of halophilic marine-derived <i>Aspergillus ruber</i>.

3 Biotech·2026
Same author

Decoding the Mutational Hierarchy of Thyroid Cancer and Associated Signaling Pathways.

Archives of medical research·2026
Same author

Photobiomodulation of 450 nm Blue Light on Human Keratinocytes, Fibroblasts, and Endothelial Cells: An In Vitro and Transcriptomic Study on Cells Involved in Wound Healing and Angiogenesis.

Biomedicines·2025
Same author

Biphasic Effects of Blue Light Irradiation on Different Drug-Resistant Bacterium and Exploration of Its Mechanism.

Biomedicines·2025
Same author

Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.

Chemical & biomedical imaging·2024

Related Experiment Video

Updated: Mar 15, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K

Obtaining miRNA-Target Interaction Information from miRWalk2.0.

Alisha Parveen1, Norbert Gretz1, Harsh Dweep1

  • 1Medical Research Center, Medical Faculty of Mannheim, University of Heidelberg, Mannheim, Germany.

Current Protocols in Bioinformatics
|September 8, 2016
PubMed
Summary

miRWalk2.0 provides the largest collection of microRNA (miRNA)-target interactions, including predicted and verified data. This resource aids researchers by offering millions of interactions between miRNAs, genes, and lncRNAs.

Keywords:
databasegene ontologiesmiRNAmiRWalk2.0micro RNApathwaystarget predictions

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

481
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.7K

Related Experiment Videos

Last Updated: Mar 15, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

481
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.7K

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • MicroRNA (miRNA)-target interactions are crucial for gene regulation.
  • Existing databases offer limited or fragmented information on these interactions.
  • A comprehensive, updated resource is needed for the scientific community.

Purpose of the Study:

  • To introduce miRWalk2.0, a comprehensive archive of miRNA-target interactions.
  • To highlight the unique features and extensive data collection of miRWalk2.0.
  • To provide a workflow for accessing miRNA-target interaction data.

Main Methods:

  • Data aggregation from multiple sources of predicted and experimentally verified miRNA-target interactions.
  • Integration of diverse identifiers for genes, miRNAs, and lncRNAs.
  • Development of a user-friendly interface for data retrieval.

Main Results:

  • miRWalk2.0 archives approximately 949 million miRNA-target interactions.
  • The database includes 11,748 miRNAs, 308,700 genes, and 68,460 lncRNAs.
  • Over 5 million unique identifiers are supported, facilitating data collection.

Conclusions:

  • miRWalk2.0 serves as a valuable, one-stop resource for miRNA-target interaction data.
  • The archive's extensive collection and features support diverse research needs.
  • Regular updates ensure the continued relevance and comprehensiveness of the database.