Related Experiment Video
Updated: Mar 15, 2026
12:31
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
14.9K
Phage Display Derived IgNAR V Region Binding Domains for Therapeutic Development
Obinna C Ubah, Caroline J Barelle1, Magdalena J Buschhaus
1Elasmogen Limited, Liberty Building, Foresterhill Road, Aberdeen, AB25 2ZP, United Kingdom.
Current Pharmaceutical Design
|September 9, 2016
Summary
Phage display technology enables the isolation of novel therapeutic and diagnostic molecules. This review highlights its crucial role in characterizing variable domain of New Antigen Receptors (VNARs) from sharks.
Area of Science:
- Biotechnology
- Immunology
- Drug Discovery
Background:
- Phage display technology is a powerful tool for generating and selecting ligands like antibody fragments, peptides, and enzymes.
- Variable domain of New Antigen Receptors (VNARs) are small, single-domain antigen-binding proteins found in cartilaginous fish, representing an ancient component of vertebrate adaptive immunity.
Purpose of the Study:
- To review the application of phage display technology in the isolation and characterization of VNAR domains.
- To highlight the potential of VNARs as therapeutic and diagnostic agents.
Main Methods:
- Phage display involves expressing recombinant proteins fused to phage coat proteins.
- Ligands are isolated based on specific binding, catalytic, or biological characteristics.
Main Results:
- Phage display has successfully identified numerous diagnostic and therapeutic molecules.
- VNARs isolated via phage display exhibit advantageous properties like small size, high stability, solubility, and target selectivity.
Conclusions:
- Phage display is instrumental in harnessing the potential of VNARs for therapeutic and diagnostic applications.
- VNARs represent a promising class of biologics due to their unique structure and properties.
