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Related Concept Videos

DNA Microarrays02:34

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
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Profiling Pre-MicroRNA and Mature MicroRNA Expressions Using a Single Microarray and Avoiding Separate Sample

Lin Gan1, Bernd Denecke2

  • 1Interdisciplinary Centre for Clinical Research Aachen, RWTH Aachen University, Pauwelstr. 30, 52074 Aachen, Germany. lgan@ukaachen.de.

Microarrays (Basel, Switzerland)
|September 9, 2016
PubMed
Summary

This study presents a novel method for pre-microRNA expression profiling using existing microarrays. It reveals insights into pre-microRNA expression and its relationship with mature microRNA in humans and mice.

Keywords:
mature microRNAmicroarraypre-microRNAprofiling

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biomarker Discovery

Background:

  • Mature microRNA (miRNA) is vital for gene expression regulation.
  • Pre-microRNAs offer potential insights into miRNA gene regulation and serve as biomarkers.
  • Pre-microRNA expression profiling remains underexplored.

Purpose of the Study:

  • To test parallel mature/pre-microRNA expression profiling on a single platform.
  • To provide a glimpse of pre-microRNA expression in humans and mice.
  • To explore the relationship between pre- and mature miRNA expression.

Main Methods:

  • Re-analysis of human and mouse miRNA profile datasets (Affymetrix miRNA 2.0 array).
  • Profiling of both mature and pre-microRNA signals from the same datasets.
  • Comparative analysis of pre- and mature miRNA expression.

Main Results:

  • Demonstrated feasibility of pre-microRNA expression profiling using current microarrays.
  • Provided initial data on pre-microRNA expression patterns in humans and mice.
  • Revealed correlations between pre- and mature miRNA expression levels.

Conclusions:

  • Current miRNA microarrays can profile pre-microRNA expression cost-effectively.
  • This approach offers a time- and resource-efficient method for pre-miRNA analysis.
  • Findings pave the way for further research into pre-miRNA roles and applications.