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Updated: Mar 15, 2026
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
A lysosome-targeted fluorescent sensor for the detection of glutathione in cells with an extremely fast response
Rong Huang1, Bei-Bei Wang, Xing-Ming Si-Tu
1State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. fljiang@whu.edu.cn yiliuchem@whu.edu.cn.
Abstract:
A highly sensitive and selective Rhodamine B-based fluorescent sensor, RhB-1, for glutathione (GSH) was easily synthesized. An extremely fast detection response time of 10 s, which is the fastest one ever reported, is achieved in aqueous solutions over a wide pH range with large enhancement of emission intensity. The sensor detects GSH in cells and selectively accumulates in lysosomes.
