Related Experiment Video
Updated: Mar 15, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
Published on: December 30, 2016
Production of Pure Aqueous 13 C-Hyperpolarized Acetate by Heterogeneous Parahydrogen-Induced Polarization
Kirill V Kovtunov1,2, Danila A Barskiy3, Roman V Shchepin3
1Laboratory of Magnetic Resonance Microimaging, International Tomography Center, SB RAS, 3A Institutskaya St., Novosibirsk, 630090, Russia. kovtunov@tomo.nsc.ru.
Abstract:
A supported metal catalyst was designed, characterized, and tested for aqueous phase heterogeneous hydrogenation of vinyl acetate with parahydrogen to produce 13 C-hyperpolarized ethyl acetate for potential biomedical applications. The Rh/TiO2 catalyst with a metal loading of 23.2 wt % produced strongly hyperpolarized 13 C-enriched ethyl acetate-1-13 C detected at 9.4 T. An approximately 14-fold 13 C signal enhancement was detected using circa 50 % parahydrogen gas without taking into account relaxation losses before and after polarization transfer by magnetic field cycling from nascent parahydrogen-derived protons to 13 C nuclei. This first observation of 13 C PHIP-hyperpolarized products over a supported metal catalyst in an aqueous medium opens up new possibilities for production of catalyst-free aqueous solutions of nontoxic hyperpolarized contrast agents for a wide range of biomolecules amenable to the parahydrogen induced polarization by side arm hydrogenation (PHIP-SAH) approach.
Related Concept Videos
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Acid Halides to Carboxylic Acids: Hydrolysis
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Polyprotic Acids
Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
![Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F59399.jpg&w=3840&q=50)
