Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular
Vladimir Z Prokopović1,2, Anna S Vikulina1,3, David Sustr1,2
1Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.
ACS Applied Materials & Interfaces
|September 9, 2016
Summary
Gold nanoparticle coatings on polymer multilayers enhance resistance to biodegradation and control molecular transport. This creates promising, durable platforms for drug delivery in cell-based applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Tissue Engineering
Background:
- Polymer multilayers mimic the extracellular matrix (ECM) for cell culture and tissue engineering.
- Hyaluronan/polylysine multilayers are investigated for their functional properties.
Purpose of the Study:
- To describe biodegradation and molecular transport in hyaluronan/polylysine multilayers coated with gold nanoparticles.
- To assess the potential of these composite systems for drug delivery applications.
Main Methods:
- Fabrication of hyaluronan/polylysine multilayers.
- Coating multilayers with gold nanoparticles.
- Investigating molecular transport using model protein lysozyme.
- Assessing biodegradation resistance.
Main Results:
- Gold nanoparticle coating creates a semipermeable barrier, controlling molecular transport.
- The nanoparticle coating enhances biodegradation resistance of the multilayers.
- Lysozyme diffusion into the multilayers occurs as both fast- and slow-diffusing populations.
- An equilibrium exists between these diffusing protein populations.
Conclusions:
- Gold nanoparticle-coated hyaluronan/polylysine multilayers offer enhanced biodegradation resistance.
- These composite systems effectively regulate molecular transport, mimicking ECM functions.
- The system shows potential as degradation-resistant drug-delivery platforms for cell-based applications.
