A semiautomatic image analyzer for cell counts in monolayers. III. Statistical considerations

The image-analyzing system described in the companion paper (Thorén and Lanke, 1989) is considered from a statistical point of view. Special attention is given to the following applications: simple cell counts, viability estimation, and toxicity estimation. Different estimators are considered, their variances are discussed, and it is shown how to compute standard errors in different situations.