Related Experiment Video

Updated: Mar 15, 2026

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
10:03

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

Published on: November 21, 2019

8.3K

Microvesicles from brain-extract-treated mesenchymal stem cells improve neurological functions in a rat model of

Ji Yong Lee1, Eiru Kim2, Seong-Mi Choi1

  • 1Institute for BioMedical Convergence, Catholic Kwandong University-International St. Mary's Hospital, Incheon 22711, Republic of Korea.

Scientific Reports
|September 10, 2016
PubMed

Insights

Mesenchymal stem cell-derived microvesicles (MVs) enhanced by brain extracts show improved therapeutic effects for ischemic stroke. These MVs promote tissue repair, reduce inflammation, and boost neurogenesis in rat models.

Area of Science:

  • Regenerative Medicine
  • Neuroscience
  • Biotechnology

Background:

  • Mesenchymal stem cells (MSCs) show therapeutic potential for ischemic stroke.
  • MSC-derived microvesicles (MVs) may offer similar benefits to MSC transplantation.
  • Optimizing MSC-MV composition could enhance stroke treatment efficacy.

Purpose of the Study:

  • To evaluate the therapeutic effects of MSC-MVs treated with normal (NBE) and stroke-injured (SBE) rat brain extracts on ischemic stroke.
  • To compare the efficacy of NBE-MSC-MVs and SBE-MSC-MVs against standard MSC-MVs.
  • To elucidate the molecular mechanisms underlying the enhanced therapeutic effects of brain-extract-treated MSC-MVs.

Main Methods:

  • Induction of permanent middle cerebral artery occlusion (pMCAO) in a rat model of ischemic stroke.
  • Preparation and characterization of three types of MSC-MVs: MSC-MVs, NBE-MSC-MVs, and SBE-MSC-MVs.
  • Assessment of functional recovery, immunohistochemical analysis, and mass spectrometry proteomic analysis.

Main Results:

  • NBE-MSC-MVs and SBE-MSC-MVs demonstrated significantly greater efficacy in ameliorating ischemic brain injury and improving functional recovery compared to MSC-MVs.
  • Similar signaling protein profiles were observed in NBE-MSC-MVs and SBE-MSC-MVs, correlating with their comparable therapeutic efficacies.
  • Brain-extract-treated MSC-MVs were found to reduce inflammation, enhance angiogenesis, and promote endogenous neurogenesis in the injured brain.

Conclusions:

  • MSC-MVs treated with brain extracts exhibit superior therapeutic potential for ischemic stroke compared to untreated MSC-MVs.
  • The enhanced efficacy is attributed to specific protein enrichments in MVs, particularly those involved in tissue repair pathways.
  • MSC-MV proteins stimulated by the brain microenvironment act as paracrine effectors, augmenting MSC therapy for stroke injury.

More Related Videos

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia14:53

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

Published on: June 26, 2020

11.2K
Author Spotlight: Advancing Glioblastoma Treatment Through Intraarterial Delivery Strategies for Oncolytic Viruses and MicroRNAs09:08

Author Spotlight: Advancing Glioblastoma Treatment Through Intraarterial Delivery Strategies for Oncolytic Viruses and MicroRNAs

Published on: March 15, 2024

3.2K

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.9K