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Updated: Aug 18, 2026

Lentivirus Production
Published on: October 3, 2009
Identification of a sequence required for efficient packaging of human immunodeficiency virus type 1 RNA into virions
A Lever1, H Gottlinger, W Haseltine
1Laboratory of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Abstract:
Sequences required for efficient packaging of human immunodeficiency virus type 1 (HIV-1) genome RNA into virus particles were identified. Deletion of 19 base pairs between the 5' long terminal repeat and the gag gene initiation codon of HIV-1 resulted in a virus markedly attenuated for replication in human T lymphocytes. The mutant virus was characterized by nearly wild-type ability to encode viral proteins and to produce virion particles. The mutant virions exhibited a significant reduction in the content of HIV-1-specific RNA. These results identify an important component of the HIV-1 packaging signal.
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