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Draft Genome Sequences of Three Northern German Epidemic Staphylococcus aureus (ST247) Strains Containing Multiple

Franziska Kleinert1, René Kallies2, Annegret Zweynert1

  • 1Institute of Medical Microbiology, Immunology and Parasitology, University of Bonn, Bonn, Germany.

Genome Announcements
|September 10, 2016
PubMed

Insights

We sequenced three multiresistant Staphylococcus aureus strains, including methicillin-resistant Staphylococcus aureus (MRSA). Findings reveal varying vancomycin susceptibility profiles in these important clinical pathogens.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Multiresistant Staphylococcus aureus, particularly methicillin-resistant S. aureus (MRSA), poses a significant global health threat.
  • Sequence type 247 (ST247) is a notable lineage associated with MRSA infections.

Purpose of the Study:

  • To report the draft genome sequences of three distinct multiresistant Staphylococcus aureus strains belonging to ST247.
  • To characterize the vancomycin susceptibility profiles of these sequenced strains.

Main Methods:

  • Whole-genome sequencing was performed on three S. aureus isolates.
  • Antimicrobial susceptibility testing, specifically for vancomycin, was conducted.

Main Results:

  • Draft genome sequences for three multiresistant S. aureus ST247 strains were generated.
  • One strain (SA1450/94) exhibited vancomycin susceptibility.
  • A clinical isolate (SA137/93A) and its laboratory mutant (SA137/93G) displayed intermediate vancomycin susceptibility.

Conclusions:

  • The genomic data provide insights into the genetic basis of antimicrobial resistance in S. aureus ST247.
  • Understanding vancomycin susceptibility variations is crucial for managing MRSA infections caused by this lineage.

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