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In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through
Juan Cao1, Shike Hou1, Hui Ding1
1Institute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force, Tianjin, China; Key Laboratory of Emergency and Disaster Medicine in Chinese People's Liberation Army (PLA), Tianjin 300162, China.
Stem Cells International
|September 10, 2016
Summary
Mesenchymal stem cells (MSCs) were tracked in rats after transplantation. These cells primarily localized to the skin
Area of Science:
- Regenerative Medicine
- Cell Biology
- Immunology
Background:
- Mesenchymal stem cells (MSCs) show therapeutic promise in clinical trials.
- Understanding MSC biodistribution is crucial for effective treatment.
- Limited data exists on the in vivo distribution of MSCs.
Purpose of the Study:
- To investigate the dynamic distribution of systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in rats.
- To determine the long-term fate and localization of BMSCs after intravenous administration.
Main Methods:
- Bioluminescence imaging (BLI) for real-time tracking of BMSCs.
- Ex vivo organ imaging to confirm distribution.
- Immunohistochemistry (IHC) and RT-PCR for histological verification.
Main Results:
- BMSCs were primarily detected in the dorsal skin, subepidermal connective tissue, and adipose tissue.
- Lungs and kidneys showed initial MSC presence, but not parenchymatous organs.
- MSCs were undetectable after 14 days, with no significant adverse effects noted besides transient neutropenia.
Conclusions:
- Allogeneic BMSCs exhibit specific homing patterns to the skin in healthy rats.
- The transient presence and localization of BMSCs provide insights into their biocharacteristics.
- Findings support further investigation into MSC behavior for therapeutic applications.
