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Updated: Mar 15, 2026

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
Published on: April 13, 2012
New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation
Fabien De Bellis1, Roger Malapa2, Valérie Kagy3
1Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, F-34398 Montpellier, France.
Premise Of The Study:
Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars.
Methods And Results:
A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single-locus markers were selected and assessed using 41 samples (39 A. altilis, one A. camansi, and one A. heterophyllus). All loci were polymorphic in A. altilis, 44 in A. camansi, and 21 in A. heterophyllus. The number of alleles per locus ranged from two to 19.
Conclusions:
The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management.
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