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Electron microscopic observation of preretinal membranes
T Yamamoto1, H Yamashita, S Hori
1Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Japanese Journal of Ophthalmology
|January 1, 1989
Summary
Electron microscopy revealed the structure of preretinal membranes, showing flat cells on the vitreous surface. These cells, when exposed to ATP and Mg2+, contracted while maintaining intercellular connections.
Area of Science:
- Ophthalmology
- Cell Biology
- Biochemistry
Background:
- Preretinal membranes can cause visual impairment due to contraction.
- The cellular mechanisms underlying preretinal membrane contraction are not fully understood.
Purpose of the Study:
- To elucidate the three-dimensional structure of preretinal membranes.
- To investigate the cellular mechanism of preretinal membrane contraction.
Main Methods:
- Observation of surgically removed preretinal membranes using various electron microscopy techniques.
- Biochemical assay involving exposure of glycerinated membranes to Adenosine Triphosphate (ATP) and Magnesium ions (Mg2+).
Main Results:
- Preretinal membranes exhibit a cellular vitreous surface with two distinct cell types (extended processes and pavement arrangement) and an acellular retinal surface.
- Internal lamellae revealed interconnected cell networks.
- ATP and Mg2+ induced contraction of vitreous-side cells, preserving intercellular connections.
Conclusions:
- Preretinal membrane contraction is mediated by the contractile behavior of vitreous-side cells.
- Intercellular connections are maintained during contraction, suggesting a coordinated cellular response.