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A cell-compatible PEO-PPO-PEO (Pluronic®)-based hydrogel stabilized through secondary structures.

Sydney Peng1, Ji-Yu Lin1, Ming-Huei Cheng2

  • 1Deparment of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.

Materials Science & Engineering. C, Materials for Biological Applications
|September 11, 2016
PubMed
Summary

Pluronic F-127 hydrogels were modified with oligo(peptides) to improve stability and cell compatibility. These enhanced Pluronic F-127 (PF127) hydrogels show improved integrity and extended in vivo residence time for drug delivery and cell culturing.

Keywords:
HydrogelPluronicPolypeptideSecondary structureThermosensitive

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Pluronic F-127 (PF127) is a thermosensitive polymer with potential bio-applications.
  • PF127 hydrogels exhibit rapid disintegration and poor cell compatibility, limiting their use.
  • Stabilizing secondary structures can enhance PF127 hydrogel integrity and cell compatibility.

Purpose of the Study:

  • To improve the integrity and cell compatibility of Pluronic F-127 hydrogels.
  • To introduce stabilizing secondary structures via oligo-alanine and oligo-phenylalanine.
  • To explore the impact of oligo(peptide) modification on PF127 hydrogel properties and performance.

Main Methods:

  • Modification of PF127 with oligo(peptides) (oligo-alanine, oligo-phenylalanine).
  • Characterization of hydrogel properties: gelation concentration/temperature, weight loss, morphology (SEM), secondary structures (CD, FT-IR), micelle core interactions (NMR).
  • Assessment of cell compatibility: chondrocyte proliferation and in vivo residence time.

Main Results:

  • Increased oligo(peptide) content decreased gelation concentration and temperature.
  • Modified PF127 hydrogels showed reduced weight loss (20% after 7 days) and enhanced interconnected morphology.
  • Spectroscopic analysis confirmed secondary structure formation and micelle core interactions.
  • Modified hydrogels supported chondrocyte proliferation and demonstrated an in vivo residence time exceeding 2 weeks.

Conclusions:

  • Oligo(peptide) modification significantly enhances the stability and cell compatibility of PF127 hydrogels.
  • The modified hydrogels offer improved structural integrity and prolonged in vivo performance.
  • These advancements broaden the application scope of PF127 hydrogels for long-term drug delivery and cell culturing.