Multifunctional soft hybrid bio-platforms based on nano-silver and natural compounds
M E Barbinta-Patrascu1, N Badea2, C Pirvu2
1University of Bucharest, Faculty of Physics, Department of Electricity and Magnetism, Solid-State Physics, and Biophysics, 405 Atomistilor Street, Bucharest-Magurele 077125, Romania.
Materials Science & Engineering. C, Materials for Biological Applications
|September 11, 2016
Summary
Novel nanohybrids combining silver nanoparticles, chitosan, lipids, and plant compounds show excellent antioxidant and antimicrobial properties. These stable, spherical nano-entities are safe for normal cells, indicating potential in biomedical applications like coatings and cancer therapy.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Development of advanced nanomaterials is crucial for innovative biomedical solutions.
- Hybrid nanostructures offer synergistic properties for enhanced performance.
- Natural compounds and nanoparticles can be combined for improved bioactivity.
Purpose of the Study:
- To synthesize novel nanohybrids using silver nanoparticles, chitosan, lipids, and plant compounds (chlorophyll a, curcumin).
- To characterize the physical, chemical, and biological properties of the synthesized nanohybrids.
- To evaluate the potential of these nanohybrids in biomedical applications, including antimicrobial coatings and cancer treatment.
Main Methods:
- A simple bottom-up strategy was employed for nanohybrid synthesis.
- Atomic Force Microscopy (AFM) and Dynamic Light Scattering (DLS) were used for size and shape analysis.
- Absorption and emission spectroscopy monitored hybrid formation, leveraging chlorophyll a's spectral signature.
- In vitro assays assessed antioxidant activity, biocidal efficacy against Escherichia coli, hemocompatibility, and cytotoxicity against HT-29 cancer cells and normal cells.
Main Results:
- Stable, spherical nanohybrids with sizes under 200nm and a zeta potential of -30.9mV were successfully synthesized.
- High antioxidant activity (96.63%) and potent biocidal properties against Escherichia coli (32mm inhibition zone) were observed.
- The nanohybrids demonstrated hemocompatibility and selective in vitro cytotoxicity against HT-29 cancer cells.
- No toxicity was observed in normal cells within the tested concentration range (5.7-17%).
Conclusions:
- The synthesized nanohybrids possess desirable physicochemical properties and significant bio-performances.
- These novel nanohybrids show great promise for applications in antimicrobial and antioxidant coatings.
- Their selective anticancer activity and safety profile make them strong candidates for cancer treatment strategies.
