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Accelerated cell-surface interlocking on plasma polymer-modified porous ceramics.

Henrike Rebl1, Birgit Finke2, Jürgen Schmidt3

  • 1Dept. of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany.

Materials Science & Engineering. C, Materials for Biological Applications
|September 11, 2016
PubMed
Summary
This summary is machine-generated.

Improving dental implant success involves enhancing osseointegration. Modifying titanium alloy surfaces with calcium phosphate and allylamine coatings promotes osteoblast cell attachment and spreading for better implant interlocking.

Keywords:
Actin cytoskeletonCell morphologyCeramicOsteoblastPlasma chemical oxidationPlasma polymer filmSurface chargeTitanium implantWater contact angle

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Osseointegration of permanent implants is critical for long-term success.
  • Bio-inert titanium alloy (Ti6Al4V) surfaces require modification to enhance biological interaction.
  • Current methods aim to improve the osteoconductive potential of implant surfaces.

Purpose of the Study:

  • To enhance the osseointegrative potential of titanium alloy surfaces.
  • To investigate the effect of a multilayer surface modification on osteoblast behavior.
  • To understand the role of surface chemistry and topography in cell attachment.

Main Methods:

  • Titanium alloy (Ti6Al4V) surfaces were modified using plasma chemical oxidation (PCO®) to create a calcium phosphate layer.
  • A cell-adhesive plasma-polymerized allylamine (PPAAm) nano film was applied to the PCO surface.
  • Human osteoblast-like MG-63 cells were cultured on the modified surfaces to assess cell response.

Main Results:

  • The PCO® process converted the native titanium oxide to a porous calcium phosphate layer (~10μm).
  • The addition of PPAAm nano films (5 and 50nm) significantly increased MG-63 cell size and filopodia formation.
  • Cells exhibited enhanced attachment by molding to the porous topography of the PPAAm-covered PCO surface.
  • Positive surface charges from PPAAm, not just hydrophilicity, were crucial for rapid cell spreading.

Conclusions:

  • The multilayer composite material of PCO® and PPAAm significantly enhances osteoblast interaction with titanium alloy surfaces.
  • This surface modification promotes cell spreading and strong attachment through physical interlocking.
  • The findings suggest a promising strategy for improving the long-term success of dental and orthopedic implants.