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The interactome of CCT complex - A computational analysis.
Aswathy Narayanan1, Dileep Pullepu1, M Anaul Kabir1
1Molecular Genetics Laboratory, School of Biotechnology, National Institute of Technology Calicut, Calicut 673601, Kerala, India.
Computational Biology and Chemistry
|September 12, 2016
Summary
The Chaperonin Containing TCP1 (CCT) protein complex plays a crucial role in cellular stress responses. Computational analysis of CCT interactomes in yeast and humans reveals its involvement in disease pathways and highlights key interacting proteins.
Area of Science:
- Molecular Biology
- Cell Biology
- Systems Biology
Background:
- The eukaryotic chaperonin, CCT (Chaperonin Containing TCP1 or TriC-TCP-1 Ring Complex), is vital for protein folding.
- Structural and functional similarities exist between yeast CCT (yCCT) and human CCT (hCCT).
- hCCT is implicated in diseases such as neurodegeneration and cancer.
Purpose of the Study:
- To investigate the stress response role of CCT.
- To computationally analyze the CCT interactome in yeast and humans.
- To identify conserved and divergent functions of CCT across species.
Main Methods:
- Comparative analysis of unique CCT-interacting protein lists from S. cerevisiae and H. sapiens.
- Gene Ontology (GO) term classification and enrichment studies.
- Interactome-wide network analysis.
Main Results:
- The CCT interactome exhibits diversity in protein classes, including WD-repeat proteins, protein kinases, and cytoskeletal proteins.
- Enrichment analysis revealed associations with disease-associated proteins and pathways.
- The study suggests cross-talk between protein synthesis, folding, and degradation machinery, involving proteasomal and ribosomal proteins.
- A network of chaperones and chaperonins, including Hsp70, functioning with CCT was identified.
Conclusions:
- CCT's stress response role is conserved across yeast and humans, with implications for human diseases.
- The CCT interactome provides insights into cellular protein homeostasis and disease mechanisms.
- WD-repeat proteins, kinases, and cytoskeletal proteins are significant interactors of CCT.
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