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Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
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Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Published on: September 14, 2019

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Presence of intracellular viruses in human Cryptosporidium isolates

Poonam Sharma, Sumeeta Khurana, Aman Sharma

    Annals of Parasitology
    |September 12, 2016
    PubMed
    Abstract

    Insights

    Intracellular viruses were detected in all Cryptosporidium isolates from immunocompromised patients and children. However, viral load did not correlate with disease severity or specific clinical symptoms like diarrhea.

    Area of Science:

    • Parasitology
    • Virology
    • Molecular Biology

    Background:

    • Cryptosporidium is a significant cause of diarrheal illness, particularly in children and immunocompromised individuals.
    • Intracellular viruses within parasites are implicated in disease pathogenesis.
    • The role of viruses in Cryptosporidium infection requires further investigation.

    Purpose of the Study:

    • To detect and quantify intracellular viruses in Cryptosporidium isolates.
    • To investigate the relationship between viral load and clinical features of cryptosporidiosis.

    Main Methods:

    • Microscopy and PCR-RFLP were used to identify and differentiate Cryptosporidium species.
    • Sequencing of the GP60 region was performed for subtyping.
    • Copy numbers of dsRNA virus and 18s rRNA were quantified using qPCR.
    • The ratio of viral copy number to 18s rRNA copy number per oocyst was calculated.

    Main Results:

    • Intracellular viruses (CSpV) were detected in all 56 Cryptosporidium isolates examined.
    • No statistically significant difference was found in the mean CSpV/RNA ratio between C. hominis and C. parvum isolates.
    • No association was observed between CSpV copy number or CSpV/RNA ratio and clinical symptoms such as diarrhea, vomiting, cough, or fever.

    Conclusions:

    • Intracellular viruses are consistently present in Cryptosporidium isolates.
    • The presence or quantity of these viruses does not appear to be associated with the clinical presentation of cryptosporidiosis in the studied population.

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