Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Identifying age-common and age-specific factors of Plasmodium infection in Nigerian children under five: Application of a cluster-aware multistage selection framework to the 2018 Nigeria Demographic and Health Survey.

PLOS global public health·2026

Same author

Field investigation of bovine leukemia virus: <i>env</i>-based PCR detection and hematologic predictors in Korean dairy herds with productivity issues.

Journal of veterinary science·2026

Same author

Case Report: Integrated imaging and molecular diagnostic approach to congenital hip dysplasia in a Hanwoo calf.

Frontiers in veterinary science·2026

Same author

Phenotypic profiles for anticancer drug responses using tumor spheroids.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Same author

Development of a customizable vascularization strategy using a modular microfluidic platform for fibroblast-free vascularized tissue.

Materials today. Bio·2026

Same author

Pilot Study: Exploring the Feasibility of Individual Voluntary Waiting Period Settings Using Postpartum Recovery Indicators in Dairy Cows.

Animals : an open access journal from MDPI·2025

Search research articles

Related Experiment Video

Updated: Mar 15, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Design methodology for a confocal imaging system using an objective microlens array with an increased working

Woojae Choi¹, Ryung Shin^1,2, Jiseok Lim^2,3

¹School of Mechanical Engineering, Yonsei University, Seoul, 03722, South Korea.

Scientific Reports

|September 13, 2016

Summary

A new design methodology for multi-optical probe confocal imaging systems offers scalability and ease of implementation. This approach achieves high resolution (0.98 μm) and a large field of view for advanced imaging applications.

More Related Videos

Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Mar 15, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Area of Science:

Optical Engineering
Microscopy
Imaging Systems

Background:

Conventional multi-optical probe confocal imaging systems face challenges with optical complexity and limited working distances.
Developing scalable and easily implementable imaging systems is crucial for advancing microscopy techniques.

Purpose of the Study:

To develop a novel design methodology for a multi-optical probe confocal imaging system.
To create a scalable and user-friendly confocal imaging system with enhanced resolving power and imaging area.

Main Methods:

A micro-objective lens module was designed using two microlens arrays and a telecentric relay optical system.
Fabrication involved backside alignment photolithography and thermal reflow processes on a glass substrate.
A 10x10 multi-optical probe array system was constructed to validate the methodology.

Main Results:

The developed system achieved a resolution of 1 μm/pixel.
It offers a 1 mm x 1 mm field of view and a 100 μm sample scanning range.
Knife-edge testing confirmed a measured lateral resolution of 0.98 μm.

Conclusions:

The proposed design methodology successfully addresses limitations of conventional systems.
The developed system demonstrates high optical resolution and a practical field of view.
This work provides a foundation for scalable and accessible confocal imaging solutions.