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Updated: Mar 15, 2026

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
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An easy-to-use and versatile method for building cell-laden microfibres.

Jérome Kalisky1,2, Jérémie Raso1,2, Claire Rigothier1,3

  • 1University of Bordeaux, Tissue Bioengineering, U1026, F-33076 Bordeaux, France.

Scientific Reports
|September 13, 2016
PubMed
Summary

Researchers developed a simple core-shell method to create stable, aligned cellular microfibers from alginate and collagen. These constructs support cell growth, orientation, and osteoblastic differentiation of stem cells.

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cellular Biology

Background:

  • Three-dimensional (3D) functional structures mimicking complex tissues are crucial for regenerative medicine.
  • Existing hydrogel microfiber fabrication methods lack control over alignment and require extensive protocol optimization.

Purpose of the Study:

  • To present a simple, scalable method for producing aligned, cell-laden microfibers using a core-shell approach.
  • To demonstrate the stability, cell behavior, and differentiation potential of these novel cellular constructs.

Main Methods:

  • A core-shell fabrication technique using calcium alginate and type I collagen was employed.
  • The alginate shell was removed after 24 hours of culture, yielding stable cellular fibers.
  • Murine bone marrow mesenchymal stem cells were cultured within the fibers.

Main Results:

  • The method produced stable and reproducible fiber-shaped cellular constructs with controlled alignment.
  • Cells preferentially distributed to the fiber surface and exhibited uniform orientation over time.
  • Mesenchymal stem cells differentiated towards the osteoblastic lineage without specific osteoinductive factors.

Conclusions:

  • This novel method offers a simple and scalable approach for fabricating cellular microfibers.
  • The resulting constructs support cell alignment and spontaneous osteoblastic differentiation, showing potential for bone tissue engineering applications.