Determination of Age-Dependent Reference Ranges for Coagulation Tests Performed Using Destiny Plus

Area of Science:

• Pediatric Hematology
• Clinical Pathology
• Hemostasis and Thrombosis

Background:

• Accurate diagnosis of pediatric hemostatic disorders requires age-specific laboratory reference ranges.
• Current adult reference ranges may not be suitable for interpreting pediatric coagulation test results.

Purpose of the Study:

• To determine age-dependent reference ranges for prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer.
• To establish distinct laboratory values for pediatric and adult populations.

Main Methods:

• A cohort of 320 volunteers across various age groups (1 month to 65 years) was studied.
• Standard laboratory tests for coagulation (PT, aPTT, fibrinogen, D-dimer) were performed.
• Nonparametric methods were used to calculate 95% distribution and 90% confidence intervals for reference ranges.

Main Results:

• Prothrombin time (PT) and activated partial thromboplastin time (aPTT) showed no significant differences among pediatric age groups but were higher in children compared to adults.
• Fibrinogen levels were significantly higher in the 6-10 year olds and adults.
• D-dimer levels were significantly lower in individuals aged 2-17 years, necessitating a separate reference range.

Conclusions:

• The findings support the concept of developmental hemostasis.
• Distinct reference ranges for adult and pediatric age groups are essential for accurate interpretation of coagulation test results.
• This study provides age-specific reference values to aid in the diagnosis and management of pediatric hemostatic disorders.