Immunocytochemical indications for neuronal co-localization of GABA and aspartate in cultured neocortex explants

The application of postembedding immunocytochemistry on serial semithin plastic sections, revealed the presence of gamma-aminobutyric acid (GABA)-positive and aspartate-positive neurons in cultured neocortex explants. GABA-positive neurons were found in all layers of the cultured cortex, whereas aspartate-positive neurons were mainly restricted to layer 6. Although exact quantification was not carried out because of the variability in aspartate immunoreactivity, colocalization of GABA and aspartate immunoreactivity could unambiguously be observed in a few neurons.