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Updated: Mar 15, 2026

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Published on: December 14, 2015
Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum
Ludovic Telley1, Christelle Cadilhac1, Jean-Michel Cioni2
1Department of Neurobiology, Institut de Génomique Fonctionnelle, CNRS, UMR5203, 34090 Montpellier, France; INSERM, U1191, 34094 Montpellier, France; Université de Montpellier, 34090 Montpellier, France; Department of Basic Neurosciences, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland.
Abstract:
Subcellular target recognition in the CNS is the culmination of a multiple-step program including axon guidance, target recognition, and synaptogenesis. In cerebellum, basket cells (BCs) innervate the soma and axon initial segment (AIS) of Purkinje cells (PCs) to form the pinceau synapse, but the underlying mechanisms remain incompletely understood. Here, we demonstrate that neuropilin-1 (NRP1), a Semaphorin receptor expressed in BCs, controls both axonal guidance and subcellular target recognition. We show that loss of Semaphorin 3A function or specific deletion of NRP1 in BCs alters the stereotyped organization of BC axon and impairs pinceau synapse formation. Further, we identified NRP1 as a trans-synaptic binding partner of the cell adhesion molecule neurofascin-186 (NF186) expressed in the PC AIS during pinceau synapse formation. These findings identify a dual function of NRP1 in both axon guidance and subcellular target recognition in the construction of GABAergic circuitry.
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