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A novel method for evaluation of oxidative stress in children with OSA
Fatih Gul1, Togay Muderris2, Gokhan Yalciner3
1Bitlis Tatvan State Hospital, Department of Otorhinolaryngology, Head and Neck Surgery, Bitlis, Turkey.
Insights
Children with obstructive sleep apnea (OSA) show higher oxidative stress. Adenotonsillectomy significantly improves this oxidative stress, as measured by thiol/disulfide homeostasis.
Area of Science:
- Pediatric Pulmonology
- Sleep Medicine
- Biochemistry
Background:
- Adenotonsillar hypertrophy is a common cause of obstructive sleep apnea (OSA) in children.
- Oxidative stress plays a role in the pathophysiology of OSA.
- Thiol/disulfide homeostasis is a novel biomarker for assessing oxidative stress.
Purpose of the Study:
- To investigate the association between adenotonsillar hypertrophy, OSA, and oxidative stress in children.
- To evaluate the impact of adenotonsillectomy (AT) on oxidative stress markers in pediatric OSA patients.
Main Methods:
- A case-control study involving 45 children with OSA and 38 healthy controls (ages 3-12).
- Overnight polysomnography was used to diagnose and grade OSA severity (mild, moderate, severe).
- Thiol/disulfide homeostasis was measured in blood samples preoperatively and one month post-AT.
Main Results:
- Children with OSA exhibited significantly higher disulfide levels and ratios compared to controls.
- Elevated disulfide levels were more pronounced in moderate to severe OSA cases.
- Postoperative thiol/disulfide homeostasis showed significant improvement compared to the preoperative period.
Conclusions:
- Pediatric OSA associated with adenotonsillar hypertrophy is linked to increased oxidative stress.
- Adenotonsillectomy appears to be an effective treatment for reducing oxidative stress in children with OSA.
Objectives:
To evaluate the role of adenotonsillar hypertrophy and the outcomes of adenotonsillectomy (AT) on oxidative stress for obstructive sleep apnea (OSA) in children using a new method; thiol/disulfide homeostasis.
Methods:
The study is consisted of 45 children with OSA and 38 healthy control subjects with similar age and sex. Children 3-12 years of age with OSA, defined as having an apnea/hypopnea index (AHI) of 5 or more in an overnight polysomnography, underwent adenotonsillectomy. OSA was classified as mild (1 ≤ AHI<10), moderate (10 ≤ AHI<20) or severe (AHI≥20). Venous blood samples were taken preoperatively and one month after surgery. The blood levels of thiol/disulfide homeostasis were assessed and compared between patients and control group, before and after adenotonsillectomy.
Results:
Body mass index (BMI), mean age and gender distribution were similar between the study and control groups. Statistically significant higher disulfide levels and ratios were found in the study group compared to the control group; in patients with moderate to severe OSA compared to mild OSA; in the preoperative period compared to postoperative period (p < 0.001, for all).
Conclusions:
The current study provides preliminary evidence between oxidative stress and OSA in children with adenotonsillar. Adenotonsillectomy for OSA may result in a dramatic improvement in oxidative stress as measured by thiol/disulfide homeostasis.
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