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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Selective, switchable fluorescent probe for heparin based on aggregation-induced emission.

Yi Jia Wang1, Lei Lin2, Xing Zhang2

  • 1MoE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China; Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States.

Analytical Biochemistry
|September 14, 2016
PubMed
Summary
This summary is machine-generated.

A new fluorescent probe, TPE-4MN, detects heparin with high sensitivity and selectivity. This aggregation-induced emission probe offers a reliable method for quantifying heparin in biological samples.

Keywords:
Aggregate induce emissionDetectionFluorescenceGlycosaminoglycansHeparin

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Heparin is a crucial anticoagulant with complex detection methods.
  • Developing sensitive and selective probes for heparin is essential for clinical diagnostics.
  • Aggregation-induced emission (AIE) probes offer unique advantages for biomolecule detection.

Purpose of the Study:

  • To synthesize and characterize a novel tetraphenylethene (TPE) derivative, TPE-4MN, as a fluorescent probe for heparin detection.
  • To investigate the sensing mechanism and evaluate the probe's performance, including sensitivity, selectivity, and limit of detection.
  • To demonstrate the probe's ability to differentiate heparin from structurally similar glycosaminoglycans.

Main Methods:

  • Synthesis of a positively charged tetraphenylethene (TPE) derivative (TPE-4MN).
  • Utilizing aggregation-induced emission (AIE) properties for fluorescence enhancement upon heparin binding.
  • Spectroscopic analysis to determine fluorescence changes, linearity, and limit of detection (LOD).
  • Investigating the interaction mechanism via ion pairing and assessing selectivity against other polysaccharides.

Main Results:

  • TPE-4MN exhibited a significant ~10-fold fluorescence enhancement upon addition of 5.0 μg/mL heparin.
  • A linear fluorescence response was observed for heparin concentrations ranging from 0-1.0 μg/mL (R=0.99988).
  • The limit of detection (LOD) for heparin was determined to be 0.75 μg/mL.
  • The probe demonstrated excellent selectivity, distinguishing heparin from heparan sulfate and other polysaccharides.

Conclusions:

  • TPE-4MN serves as an effective fluorescent probe for sensitive and selective heparin detection based on AIE.
  • The ion pairing interaction mechanism underpins the probe's response to heparin.
  • This AIE-based probe offers a promising tool for the quantification and differentiation of heparin in complex biological matrices.