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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Development and characterization of a monoclonal antibody against Pseudorabies virus glycoprotein B and its application in tracking viral infection.

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Updated: Mar 15, 2026

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
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Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system.

Yan-Dong Tang1, Ji-Ting Liu2, Tong-Yun Wang1

  • 1State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.

Virus Research
|September 14, 2016
PubMed
Summary

New CRISPR-Cas9 gene editing rapidly attenuates pseudorabies virus (PRV) variant strains. This creates a safe, effective PRV vaccine candidate offering protection against emerging PRV outbreaks.

Keywords:
AttenuationCRISPR/Cas9Pseudorabies virusVaccine

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Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Vaccine Development

Background:

  • Pseudorabies virus (PRV) variant strains, belonging to genotype II, are causing outbreaks in China.
  • The existing Bartha-K61 vaccine offers insufficient protection against these emergent PRV variants.

Purpose of the Study:

  • To develop a rapid method for attenuating current epidemic PRV strains using CRISPR-Cas9 technology.
  • To create a safe and immunogenic PRV vaccine candidate for effective PRV control.

Main Methods:

  • Utilized the CRISPR-Cas9 system for precise genome editing of PRV.
  • Developed a triple gene-inactivated (gE/gI/TK) HeN1 PRV strain.
  • Evaluated PRV attenuation and immunogenicity in a mouse model.

Main Results:

  • The modified PRV strain was demonstrated to be fully attenuated in mice.
  • The attenuated PRV strain successfully induced protective immunity against a parental PRV challenge.
  • CRISPR-Cas9 technology enabled rapid PRV attenuation.

Conclusions:

  • CRISPR-Cas9 technology offers a rapid and effective approach for PRV attenuation.
  • This method is crucial for controlling PRV, especially in response to emerging variant strains.
  • The developed attenuated PRV strain shows promise as a vaccine candidate.