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Updated: Mar 15, 2026
MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
Differential expression of microRNAs of Litopenaeus vannamei in response to different virulence WSSV infection
Xinying Sun1, Qing-Hui Liu2, Bing Yang2
1Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Abstract:
WSSV is one of the most harmful pathogeny in the pacific white shrimp, and genetic variations caused the strains of different virulence. MicroRNAs (miRNAs) involved in the regulation of virus defense. To understand the different virulence of WSSV on miRNA expression in Litopeneaus vannamei, the deep sequencing was performed to compare two small RNA libraries prepared from hepatopancreas of Litopeneaus vannamei infected with normal-virulence or low-virulence WSSV. Approximately 29,398,623 raw reads from normal-virulence library and 35,291,803 raw reads from low-virulence library were obtained. There were about 37 miRNAs homologs identified. Sixteen miRNAs were significantly up-regulated and twenty-one miRNAs were significantly down-regulated in normal-virulence infection library compared with low-virulence infection library. Of these, Igi-miR-1175-3p was the most significant different miRNA, followed by bmo-miR-1175-3p and ipu-miR-26b, respectively. The putative target genes for differentially expressed miRNAs were concerned with biological processes, signal meditated, cell differentiation and apoptosis, immune recognition and other more functions. The results will help to understand the miRNAs response to different virulence WSSV infection.
Insights
This study reveals how microRNAs (miRNAs) change in shrimp infected with different White Spot Syndrome Virus (WSSV) strains. Understanding these miRNA shifts helps explain WSSV virulence variations.
Area of Science:
- Aquatic Animal Health
- Molecular Biology
- Virology
Background:
- White Spot Syndrome Virus (WSSV) is a major pathogen affecting Pacific white shrimp (Litopeneaus vannamei).
- Genetic variations in WSSV strains lead to differences in virulence.
- MicroRNAs (miRNAs) play a crucial role in regulating antiviral defense mechanisms.
Purpose of the Study:
- To investigate the differential expression of miRNAs in Litopeneaus vannamei in response to WSSV infections of varying virulence.
- To identify specific miRNAs and their target genes associated with WSSV virulence in shrimp.
Main Methods:
- Deep sequencing of small RNA libraries from shrimp hepatopancreas infected with normal-virulence and low-virulence WSSV strains.
- Bioinformatic analysis to identify and quantify differentially expressed miRNAs.
- Prediction of target genes for identified miRNAs.
Main Results:
- A total of approximately 64.7 million raw reads were generated from the two libraries.
- 37 miRNA homologs were identified, with 16 significantly upregulated and 21 significantly downregulated in the normal-virulence WSSV infection.
- Igi-miR-1175-3p, bmo-miR-1175-3p, and ipu-miR-26b were among the most significantly differentially expressed miRNAs.
- Predicted target genes are involved in crucial cellular processes including immune response, signal transduction, cell differentiation, and apoptosis.
Conclusions:
- Differential miRNA expression patterns correlate with WSSV virulence in Litopeneaus vannamei.
- Specific miRNAs likely modulate the host's response to WSSV, influencing disease outcome.
- These findings provide insights into the molecular mechanisms underlying WSSV pathogenesis and host-virus interactions.
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